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关注:1
2013-05-23 12:21
求翻译:(PBS) plus 500 l defibrinated sheep blood (Northeast Laboratories Services, Winslow, ME), and cells were pelleted by centrifugation at 9,000 rpm (6,605 g) for 4 min. The tubes were incubated for 18 h, and the cell wall debris removed by centrifugation at 13,000 rpm. The amount of heme present in cleared cell lysate 是什么意思?![]() ![]() (PBS) plus 500 l defibrinated sheep blood (Northeast Laboratories Services, Winslow, ME), and cells were pelleted by centrifugation at 9,000 rpm (6,605 g) for 4 min. The tubes were incubated for 18 h, and the cell wall debris removed by centrifugation at 13,000 rpm. The amount of heme present in cleared cell lysate
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2013-05-23 12:21:38
(PBS),加上500升去纤维绵羊血(东北实验室服务,斯罗,我),细胞沉淀,离心4分钟9000转(6,605克)。管培养18小时,并在13000转离心除去细胞壁碎片。在清除细胞裂解液中的血红素由od415确定目前的数量作为衡量的红血细胞裂解和归到细菌细胞密度(OD600)。
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2013-05-23 12:23:18
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2013-05-23 12:24:58
(PBS)加上500 l defibrinated绵羊血液(东北实验室业务, Winslow,我),并且细胞由离心法射击在9,000转每分钟(6,605 g) 4 min。 管为18 h被孵化了,并且细胞壁残骸由离心法去除了在13,000转每分钟。 相当数量heme当前在被清除的细胞lysate一样坚定由OD415使用了象红血球病势渐退措施并且正常化了到细菌细胞密度(OD600)。
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2013-05-23 12:26:38
(PBS) 加 500 l 菌种保存羊血 (东北实验室服务、 温思劳,ME) 和单元格被颗粒用离心法在 4 分钟的 9,000 rpm (6,605 g)。管子被孵化 18 h,和删除用离心法在 13,000 rpm 的细胞壁碎片。目前已清除细胞是由 OD415 中血红素的量是用作血红细胞溶解的措施和规范化为细菌细胞密度 (OD600)。
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2013-05-23 12:28:18
(PBS) plus 500 l defibrinated sheep blood (Northeast Laboratories Services, Winslow, ME), and cells were pelleted by centrifugation at 9,000 rpm (6,605 g) for 4 min.管子为 18 h 被孵卵,以及在 13,000 rpm 处被离心分离撤销的细胞墙碎片。在被弄清楚的细胞中的数量的 heme 礼物 lysate 如通过 OD415 决定用作红血球消散的一种手段和到细菌的细胞密集正常化 ( OD600 )。
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